Enhancement of co-production of lutein and protein in Chlorella sorokiniana FZU60 using different bioprocess operation strategies

Abstract Co-production of multiple compounds is an efficient approach to enhance the economic feasibility microalgae-based metabolites production. In this study, Chlorella sorokiniana FZU60 was cultivated under different bioprocess strategies co-production lutein and protein. Results showed that both protein content (7.72 538.06 mg/g, respectively) were highest at onset nitrogen deficiency batch cultivation. Semi-batch III strategy, with 75% microalgal culture replacement by fresh medium, obtained similar content, productivity, yield as cultivation, demonstrating it can be used for stable continuous Fed-batch II feeding 1/3 modified BG11 achieved super-high (28.81 1592.77 mg/L, respectively), thus high-output Besides, two-stage combining light intensity shift semi-batch gained extremely high productivity (15.31 1080.41 mg/L/day, thereby a good option high-efficiency Moreover, fed-batch strategy high-quality protein, are promising in C. commercial application.